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goat anti mouse cd147 polyclonal antibody (Santa Cruz Biotechnology)

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Santa Cruz Biotechnology goat anti mouse cd147 polyclonal antibody

Figure 2. <t>CD147</t> specific shRNA results in the reduction of CD147 mRNA and protein levels in HT29 cells. The expression levels of CD147, MCT1 and MCT4 in HT29 cells after CD147 silencing. Relative mRNA levels were analysed by quantitative RT-PCR. β-actin was used as normalization control. As shown in (A) and (C), the mRNA expression of CD147 and MCT1 were significantly downregulated (p<0.01) by HT29/shRNA compared with the control group in HT29 cells. (B) Τhe mRNA expression of MCT4 had no significant change (p>0.05). The graphs are representative of three separate experiments. (D) The CD147, MCT1 and MCT4 protein expression levels by western blotting. The control group used β-actin. The results show that the protein expression levels of CD147 and MCT1 were significantly downregulated by HT29/shRNA in cells (p<0.01). There was no significant change of MCT4 protein expression (p>0.05).

Goat Anti Mouse Cd147 Polyclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1213 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/goat anti mouse cd147 polyclonal antibody/product/Santa Cruz Biotechnology
Average 96 stars, based on 1213 article reviews

goat anti mouse cd147 polyclonal antibody - by Bioz Stars, 2026-03

96/100 stars

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1) Product Images from "Downregulation of CD147 expression by RNA interference inhibits HT29 cell proliferation, invasion and tumorigenicity in vitro and in vivo."

Article Title: Downregulation of CD147 expression by RNA interference inhibits HT29 cell proliferation, invasion and tumorigenicity in vitro and in vivo.

Journal: International journal of oncology

doi: 10.3892/ijo.2013.2108

Figure 2. CD147 specific shRNA results in the reduction of CD147 mRNA and protein levels in HT29 cells. The expression levels of CD147, MCT1 and MCT4 in HT29 cells after CD147 silencing. Relative mRNA levels were analysed by quantitative RT-PCR. β-actin was used as normalization control. As shown in (A) and (C), the mRNA expression of CD147 and MCT1 were significantly downregulated (p<0.01) by HT29/shRNA compared with the control group in HT29 cells. (B) Τhe mRNA expression of MCT4 had no significant change (p>0.05). The graphs are representative of three separate experiments. (D) The CD147, MCT1 and MCT4 protein expression levels by western blotting. The control group used β-actin. The results show that the protein expression levels of CD147 and MCT1 were significantly downregulated by HT29/shRNA in cells (p<0.01). There was no significant change of MCT4 protein expression (p>0.05).

Figure Legend Snippet: Figure 2. CD147 specific shRNA results in the reduction of CD147 mRNA and protein levels in HT29 cells. The expression levels of CD147, MCT1 and MCT4 in HT29 cells after CD147 silencing. Relative mRNA levels were analysed by quantitative RT-PCR. β-actin was used as normalization control. As shown in (A) and (C), the mRNA expression of CD147 and MCT1 were significantly downregulated (p<0.01) by HT29/shRNA compared with the control group in HT29 cells. (B) Τhe mRNA expression of MCT4 had no significant change (p>0.05). The graphs are representative of three separate experiments. (D) The CD147, MCT1 and MCT4 protein expression levels by western blotting. The control group used β-actin. The results show that the protein expression levels of CD147 and MCT1 were significantly downregulated by HT29/shRNA in cells (p<0.01). There was no significant change of MCT4 protein expression (p>0.05).

Techniques Used: shRNA, Expressing, Quantitative RT-PCR, Control, Western Blot

Figure 3. Gelatin zymography analysis of the activity of MMP-2 and MMP-9 in HT29 cells after CD147 silencing. Cells were incubated for 24 h and con ditioned media were used for the measurement of MMP-2 and MMP-9 protein levels by gelatin zymography. (A) Images of the MMP-2 and MMP-9 bands representative of three independent experiments. (B) Quantitative analysis of the MMP-2 bands. *p<0.01 compared with HT29 and HT29/shRNA-control. (C) Quantitative analysis of the MMP-9 bands. *p<0.01 compared with HT29.

Figure Legend Snippet: Figure 3. Gelatin zymography analysis of the activity of MMP-2 and MMP-9 in HT29 cells after CD147 silencing. Cells were incubated for 24 h and con ditioned media were used for the measurement of MMP-2 and MMP-9 protein levels by gelatin zymography. (A) Images of the MMP-2 and MMP-9 bands representative of three independent experiments. (B) Quantitative analysis of the MMP-2 bands. *p<0.01 compared with HT29 and HT29/shRNA-control. (C) Quantitative analysis of the MMP-9 bands. *p<0.01 compared with HT29.

Techniques Used: Zymography, Activity Assay, Incubation, shRNA, Control

Figure 4. Intracellular lactate analysis in HT29 cells after CD147 silencing. The data show that the intracellular lactate concentration after transfection with HT29/shRNA was increased, *p<0.01 compared with HT29.

Figure Legend Snippet: Figure 4. Intracellular lactate analysis in HT29 cells after CD147 silencing. The data show that the intracellular lactate concentration after transfection with HT29/shRNA was increased, *p<0.01 compared with HT29.

Techniques Used: Concentration Assay, Transfection, shRNA

Figure 6. Invasive ability of HT29 cells on Matrigel after CD147 silencing. (A) Crystal violet staining results of lower surface filters show the cells invading the Matrigel (x400). (B) The number of cells that invaded through the chamber was evaluated in 3 fields for each experimental group and averaged. The invading cells of each experimental group was counted as the average of the sum of 10 fields of vision under a microscope. *p<0.05 compared with HT29.

Figure Legend Snippet: Figure 6. Invasive ability of HT29 cells on Matrigel after CD147 silencing. (A) Crystal violet staining results of lower surface filters show the cells invading the Matrigel (x400). (B) The number of cells that invaded through the chamber was evaluated in 3 fields for each experimental group and averaged. The invading cells of each experimental group was counted as the average of the sum of 10 fields of vision under a microscope. *p<0.05 compared with HT29.

Techniques Used: Staining, Microscopy

Figure 7. Multidrug chemosensitivity analysis in HT29 cells after CD147 silencing. Cells were treated with cisplatin with varying concentrations: 0.1, 1 and 10 µM for 48 h. Cell viability was determined by the WST-8 assay. CD147 silencing significantly increased the chemosensitivity of HT29 cells to cisplatin at 0.1, 1 and 10 µM compared with the control groups (p<0.01). These experiments were repeated in three separate experiments. Cells were treated with paclitaxel, gemcitabine or oxaliplatin at varying concentrations: 0.1, 1 and 10 µM for 48 h and determined by the WST-8 assay. There was no significant change of the chemosensitivity induced by CD147 silencing to paclitaxel, gemcitabine, and oxaliplatin in HT29 cells (p>0.05).

Figure Legend Snippet: Figure 7. Multidrug chemosensitivity analysis in HT29 cells after CD147 silencing. Cells were treated with cisplatin with varying concentrations: 0.1, 1 and 10 µM for 48 h. Cell viability was determined by the WST-8 assay. CD147 silencing significantly increased the chemosensitivity of HT29 cells to cisplatin at 0.1, 1 and 10 µM compared with the control groups (p<0.01). These experiments were repeated in three separate experiments. Cells were treated with paclitaxel, gemcitabine or oxaliplatin at varying concentrations: 0.1, 1 and 10 µM for 48 h and determined by the WST-8 assay. There was no significant change of the chemosensitivity induced by CD147 silencing to paclitaxel, gemcitabine, and oxaliplatin in HT29 cells (p>0.05).

Techniques Used: Control

Figure 8. CD147 specific shRNA results on tumor formation in vivo and the expression of CD147 in tumor tissues. (A) The average size of the tumor in each group was measured every 5 days, by the formula: volume = 1/2 x (length x width2). *p<0.01 compared with HT29 group. (B) Immunohistochemistry staining in tumors to detect the CD147 protein expression (x400). The CD147 protein expression is shown in three different groups (x400). *p<0.01 compared with HT29 group. The data were obtained from three independent experiments. Histological analysis with H&E staining was performed in implanted tumors (x400).

Figure Legend Snippet: Figure 8. CD147 specific shRNA results on tumor formation in vivo and the expression of CD147 in tumor tissues. (A) The average size of the tumor in each group was measured every 5 days, by the formula: volume = 1/2 x (length x width2). *p<0.01 compared with HT29 group. (B) Immunohistochemistry staining in tumors to detect the CD147 protein expression (x400). The CD147 protein expression is shown in three different groups (x400). *p<0.01 compared with HT29 group. The data were obtained from three independent experiments. Histological analysis with H&E staining was performed in implanted tumors (x400).

Techniques Used: shRNA, In Vivo, Expressing, Immunohistochemistry, Staining

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Journal: Reproduction (Cambridge, England)

Article Title: Expression of Basigin in Reproductive Tissues of Oestrogen Receptor-α or –β Null Mice

doi: 10.1530/REP-10-0069

Figure Lengend Snippet: Table 1

Article Snippet: After blocking with 5% normal rabbit serum in PBS for 20 minutes, sections were incubated with 2 μg/ml goat polyclonal antibody against mouse basigin (R&D, Minneapolis, MN, USA) at 4°C overnight.

Techniques: Expressing, Immunohistochemistry

Journal: International journal of oncology

Article Title: Downregulation of CD147 expression by RNA interference inhibits HT29 cell proliferation, invasion and tumorigenicity in vitro and in vivo.

doi: 10.3892/ijo.2013.2108

Figure Lengend Snippet: Figure 2. CD147 specific shRNA results in the reduction of CD147 mRNA and protein levels in HT29 cells. The expression levels of CD147, MCT1 and MCT4 in HT29 cells after CD147 silencing. Relative mRNA levels were analysed by quantitative RT-PCR. β-actin was used as normalization control. As shown in (A) and (C), the mRNA expression of CD147 and MCT1 were significantly downregulated (p<0.01) by HT29/shRNA compared with the control group in HT29 cells. (B) Τhe mRNA expression of MCT4 had no significant change (p>0.05). The graphs are representative of three separate experiments. (D) The CD147, MCT1 and MCT4 protein expression levels by western blotting. The control group used β-actin. The results show that the protein expression levels of CD147 and MCT1 were significantly downregulated by HT29/shRNA in cells (p<0.01). There was no significant change of MCT4 protein expression (p>0.05).

Article Snippet: Immunohistochemistry analysis used goat anti-mouse CD147 polyclonal antibody (1:50 dilution, Santa Cruz Biotechnology) to detect CD147 protein expression.

Techniques: shRNA, Expressing, Quantitative RT-PCR, Control, Western Blot

Journal: International journal of oncology

Article Title: Downregulation of CD147 expression by RNA interference inhibits HT29 cell proliferation, invasion and tumorigenicity in vitro and in vivo.

doi: 10.3892/ijo.2013.2108

Figure Lengend Snippet: Figure 3. Gelatin zymography analysis of the activity of MMP-2 and MMP-9 in HT29 cells after CD147 silencing. Cells were incubated for 24 h and con ditioned media were used for the measurement of MMP-2 and MMP-9 protein levels by gelatin zymography. (A) Images of the MMP-2 and MMP-9 bands representative of three independent experiments. (B) Quantitative analysis of the MMP-2 bands. *p<0.01 compared with HT29 and HT29/shRNA-control. (C) Quantitative analysis of the MMP-9 bands. *p<0.01 compared with HT29.

Article Snippet: Immunohistochemistry analysis used goat anti-mouse CD147 polyclonal antibody (1:50 dilution, Santa Cruz Biotechnology) to detect CD147 protein expression.

Techniques: Zymography, Activity Assay, Incubation, shRNA, Control

Journal: International journal of oncology

Article Title: Downregulation of CD147 expression by RNA interference inhibits HT29 cell proliferation, invasion and tumorigenicity in vitro and in vivo.

doi: 10.3892/ijo.2013.2108

Figure Lengend Snippet: Figure 4. Intracellular lactate analysis in HT29 cells after CD147 silencing. The data show that the intracellular lactate concentration after transfection with HT29/shRNA was increased, *p<0.01 compared with HT29.

Article Snippet: Immunohistochemistry analysis used goat anti-mouse CD147 polyclonal antibody (1:50 dilution, Santa Cruz Biotechnology) to detect CD147 protein expression.

Techniques: Concentration Assay, Transfection, shRNA

Journal: International journal of oncology

Article Title: Downregulation of CD147 expression by RNA interference inhibits HT29 cell proliferation, invasion and tumorigenicity in vitro and in vivo.

doi: 10.3892/ijo.2013.2108

Figure Lengend Snippet: Figure 6. Invasive ability of HT29 cells on Matrigel after CD147 silencing. (A) Crystal violet staining results of lower surface filters show the cells invading the Matrigel (x400). (B) The number of cells that invaded through the chamber was evaluated in 3 fields for each experimental group and averaged. The invading cells of each experimental group was counted as the average of the sum of 10 fields of vision under a microscope. *p<0.05 compared with HT29.

Article Snippet: Immunohistochemistry analysis used goat anti-mouse CD147 polyclonal antibody (1:50 dilution, Santa Cruz Biotechnology) to detect CD147 protein expression.

Techniques: Staining, Microscopy

Journal: International journal of oncology

Article Title: Downregulation of CD147 expression by RNA interference inhibits HT29 cell proliferation, invasion and tumorigenicity in vitro and in vivo.

doi: 10.3892/ijo.2013.2108

Figure Lengend Snippet: Figure 7. Multidrug chemosensitivity analysis in HT29 cells after CD147 silencing. Cells were treated with cisplatin with varying concentrations: 0.1, 1 and 10 µM for 48 h. Cell viability was determined by the WST-8 assay. CD147 silencing significantly increased the chemosensitivity of HT29 cells to cisplatin at 0.1, 1 and 10 µM compared with the control groups (p<0.01). These experiments were repeated in three separate experiments. Cells were treated with paclitaxel, gemcitabine or oxaliplatin at varying concentrations: 0.1, 1 and 10 µM for 48 h and determined by the WST-8 assay. There was no significant change of the chemosensitivity induced by CD147 silencing to paclitaxel, gemcitabine, and oxaliplatin in HT29 cells (p>0.05).

Article Snippet: Immunohistochemistry analysis used goat anti-mouse CD147 polyclonal antibody (1:50 dilution, Santa Cruz Biotechnology) to detect CD147 protein expression.

Techniques: Control

Journal: International journal of oncology

Article Title: Downregulation of CD147 expression by RNA interference inhibits HT29 cell proliferation, invasion and tumorigenicity in vitro and in vivo.

doi: 10.3892/ijo.2013.2108

Figure Lengend Snippet: Figure 8. CD147 specific shRNA results on tumor formation in vivo and the expression of CD147 in tumor tissues. (A) The average size of the tumor in each group was measured every 5 days, by the formula: volume = 1/2 x (length x width2). *p<0.01 compared with HT29 group. (B) Immunohistochemistry staining in tumors to detect the CD147 protein expression (x400). The CD147 protein expression is shown in three different groups (x400). *p<0.01 compared with HT29 group. The data were obtained from three independent experiments. Histological analysis with H&E staining was performed in implanted tumors (x400).

Article Snippet: Immunohistochemistry analysis used goat anti-mouse CD147 polyclonal antibody (1:50 dilution, Santa Cruz Biotechnology) to detect CD147 protein expression.

Techniques: shRNA, In Vivo, Expressing, Immunohistochemistry, Staining

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